3-phosphoinositide-dependent protein kinase 1
Also known as: PDK1, PDPK1
Function
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), TSSK3, protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation (By similarity). Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells (By similarity). Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells (By similarity). Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response (By similarity). Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses (By similarity). Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages (By similarity).
Classification
- Family (Pfam)
- PF14593 PH_3, PF00069 Pkinase
- InterPro
- Kinase-like_dom_sf, PDK1-typ_PH, PDPK1, PH-like_dom_sf, Prot_kinase_dom, Protein_kinase_ATP_BS, Ser/Thr_kinase_AS, Ser_Thr_kinase_AGC
- Functional cluster
- Homeobox & Zinc-Finger Transcription Factors
Experimental structures · PDB · 70
- 1H1W X-ray 2.00A
- 1OKY X-ray 2.30A
- 1OKZ X-ray 2.51A
- 1UU3 X-ray 1.70A
- 1UU7 X-ray 1.90A
- 1UU8 X-ray 2.50A
- 1UU9 X-ray 1.95A
- 1UVR X-ray 2.81A
- 1W1D X-ray 1.50A
- 1W1G X-ray 1.45A
- 1W1H X-ray 1.45A
- 1Z5M X-ray 2.17A
- … and 58 more
A predicted model is available from AlphaFold.
Gene Ontology · 45
- GO:0042995 cell projection
- GO:0005737 cytoplasm
- GO:0031410 cytoplasmic vesicle
- GO:0005829 cytosol
- GO:0005925 focal adhesion
- GO:0005634 nucleus
- GO:0005886 plasma membrane
- GO:0014069 postsynaptic density
- GO:0004676 3-phosphoinositide-dependent protein kinase activity
- GO:0005524 ATP binding
- GO:0016004 phospholipase activator activity
- GO:0043274 phospholipase binding
- GO:0106310 protein serine kinase activity
- GO:0004674 protein serine/threonine kinase activity
- GO:0030036 actin cytoskeleton organization
- GO:0019722 calcium-mediated signaling
- GO:0016477 cell migration
- GO:0071364 cellular response to epidermal growth factor stimulus
- GO:0032869 cellular response to insulin stimulus
- GO:0006351 DNA-templated transcription
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0097191 extrinsic apoptotic signaling pathway
- GO:0008286 insulin receptor signaling pathway
- GO:0048009 insulin-like growth factor receptor signaling pathway
- GO:0035556 intracellular signal transduction
- GO:0141124 intracellular signaling cassette
- GO:0010667 negative regulation of cardiac muscle cell apoptotic process
- GO:2000352 negative regulation of endothelial cell apoptotic process
- GO:0034122 negative regulation of toll-like receptor signaling pathway
- GO:0030512 negative regulation of transforming growth factor beta receptor signaling pathway
- GO:0043491 phosphatidylinositol 3-kinase/protein kinase B signal transduction
- GO:0045766 positive regulation of angiogenesis
- GO:0043536 positive regulation of blood vessel endothelial cell migration
- GO:0051897 positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
- GO:1903078 positive regulation of protein localization to plasma membrane
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
- GO:1903672 positive regulation of sprouting angiogenesis
- GO:1905564 positive regulation of vascular endothelial cell proliferation
- GO:0046777 protein autophosphorylation
- GO:0006468 protein phosphorylation
- GO:0043122 regulation of canonical NF-kappaB signal transduction
- GO:0043304 regulation of mast cell degranulation
- GO:0031295 T cell costimulation
- GO:0003323 type B pancreatic cell development
- GO:0097700 vascular endothelial cell response to laminar fluid shear stress
Drugs targeting this protein · 2
Related proteins · sequence + function similarity
- 3-phosphoinositide-dependent protein kinase 1 0.87
- Putative 3-phosphoinositide-dependent protein kinase 2 0.87
- 3-phosphoinositide-dependent protein kinase 1 0.86
- Serine/threonine-protein kinase Sgk1 0.81
- Ribosomal protein S6 kinase alpha-5 0.81
- Serine/threonine-protein kinase Sgk1 0.81
- RAC family serine/threonine-protein kinase homolog 0.81
- Serine/threonine-protein kinase Sgk1 0.81
- Serine/threonine-protein kinase Sgk1 0.80
- Serine/threonine-protein kinase Sgk1 0.80
- Serine/threonine-protein kinase Sgk2 0.80
- Serine/threonine-protein kinase Sgk2 0.80
Co-cited proteins · studied together in the literature
- 3-phosphoinositide-dependent protein kinase 1 1 shared papers
- 3-phosphoinositide-dependent protein kinase 1 1 shared papers
- Serine/threonine-protein kinase N2 2 shared papers
- Serine-threonine kinase receptor-associated protein 1 shared papers
- 3-phosphoinositide-dependent protein kinase 1 1 shared papers
- Growth factor receptor-bound protein 14 1 shared papers
- Ubiquitin carboxyl-terminal hydrolase 4 1 shared papers
- GATOR1 complex protein NPRL2 1 shared papers
- cAMP-dependent protein kinase catalytic subunit alpha 1 shared papers
- Protein kinase C zeta type 1 shared papers
- Ribosomal protein S6 kinase beta-1 1 shared papers
- Serine/threonine-protein kinase Sgk3 1 shared papers
Literature · 43 cited papers
- An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics · 2014
- Substrate-selective inhibition of protein kinase PDK1 by small compounds that bind to the PIF-pocket allosteric docking site. Chem. Biol. · 2012
- PDK1 phosphorylation at Thr354 by murine protein serine/threonine kinase 38 contributes to the negative regulation of PDK1 activity. J. Biol. Chem. · 2012
- Ubiquitin-specific protease 4 inhibits mono-ubiquitination of the master growth factor signaling kinase PDK1. PLoS ONE · 2012
- Regulation of 3-phosphoinositide-dependent protein kinase 1 activity by homodimerization in live cells. Sci. Signal. · 2010
- Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci. Signal. · 2009
- The nuclear localization of 3'-phosphoinositide-dependent kinase-1 is dependent on its association with the protein tyrosine phosphatase SHP-1. Cell. Signal. · 2009
- IGF-I regulated phosphorylation and translocation of PDK-1 in neurons. NeuroReport · 2009
- The C-terminus of PRK2/PKNgamma is required for optimal activation by RhoA in a GTP-dependent manner. Arch. Biochem. Biophys. · 2008
- Dissecting the role of the 3-phosphoinositide-dependent protein kinase-1 (PDK1) signalling pathways. Cell Cycle · 2008
- TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling. Cancer Sci. · 2008
- Regulation of 3-phosphoinositide-dependent protein kinase-1 (PDK1) by Src involves tyrosine phosphorylation of PDK1 and Src homology 2 domain binding. J. Biol. Chem. · 2008
- … and 31 more in the literature graph